WB, IHC, ELISA
Blue Ice or 4ºC
Human, Mouse, Rat
Peptide Affinity Purified
For in vitro research use only.
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
Detects endogenous levels of c-Jun only when phosphorylated at serine 63 (pSer63).
Synthetic phospho-peptide from human c-Jun around the phosphorylation site of serine 63. (pSer63).
WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Nucleus, Cytosol, Nuclear Chromosome, Nuclear Euchromatin, Nucleoplasm, Transcription Factor Ap-1 Complex, Transcriptional Repressor Complex
A 1:500 dilution of SPC-1361 was sufficient for detection of c-Jun (pSer63) in 10 µg of mouse fibroblast cell line (nih 3t3) lysates by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Domain Families, Hlh, Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, SMADs, Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Transcription Factors, Cancer, Tumor Biomarkers, Oncoproteins, Oncoprotein Transcription Factors, Immunology, Innate Immunity, TLR Signaling
V jun sarcoma virus 17 oncogene homolog (avian) Antibody, Proto oncogene c jun Antibody, Jun Activation Domain Binding Protein Antibody, Activator protein 1 Antibody, V-jun avian sarcoma virus 17 oncogene homolog Antibody, AP 1 Antibody, Transcription Factor AP 1 Antibody, Transcription factor AP-1 Antibody, JUN protein Antibody, cJun Antibody, vJun Avian Sarcoma Virus 17 Oncogene Homolog Antibody, Proto oncogene cJun Antibody, Enhancer Binding Protein AP1 Antibody, Proto-oncogene c-jun Antibody, V jun sarcoma virus 17 oncogene homolog Antibody, p39 Antibody, Transcription Factor AP1 Antibody, JUN_HUMAN Antibody, V jun avian sarcoma virus 17 oncogene homolog Antibody, Oncogene JUN Antibody, JUN Antibody, Jun proto oncogene Antibody, JUNC Antibody, Jun oncogene Antibody, AP1 Antibody
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.