WB IHC IP
~36, 43, 48 kDa
Polyclonals and antibodies
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100
For research use only, not for use in diagnostic procedure.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Synthetic peptide, corresponding to amino acids 201-250 of Human c-Jun.
c-Jun (G237) polyclonal antibody detects endogenous levels of c-Jun protein.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
JUN; p39; V-jun avian sarcoma virus 17 oncogene homolog; Proto-oncogene c-Jun; AP1; Activator protein 1; Transcription factor AP-1
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Western blot (WB) analysis of c-Jun (G237) pAb at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:MCF-7 whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug)