c-Jun (phospho-Y170) polyclonal antibody

BS4050-50ul

50ul

318 EUR

P05412

WB IHC

anticorps

~ 43, 48 kDa

Human,Mouse,Rat

Polyclonals and antibodies

WB: 1:500~1:1000 IHC: 1:50~1:200

For research use only, not for use in diagnostic procedure.

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Tyrosine 170.

p-c-Jun (Y170) polyclonal antibody detects endogenous levels of c-Jun protein only when phosphorylated at Tyr170.

JUN; p39; V-jun avian sarcoma virus 17 oncogene homolog; Proto-oncogene c-Jun; AP1; Activator protein 1; Transcription factor AP-1

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Western blot (WB) analysis of p-c-Jun (Y170) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:The Kidney tissue lysate of Rat(40ug) Lane4:The Kidney tissue lysate of Mouse(40ug)

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.