WB, IHC, ELISA
Blue Ice or 4ºC
Human, Mouse, Rat
Peptide Affinity Purified
For in vitro research use only.
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
Detects endogenous levels of c-Jun only when phosphorylated at serine 63 (pSer63).
Synthetic phospho-peptide from human c-Jun around the phosphorylation site of serine 63. (pSer63).
WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Nucleus, Cytosol, Nuclear Chromosome, Nuclear Euchromatin, Nucleoplasm, Transcription Factor Ap-1 Complex, Transcriptional Repressor Complex
A 1:500 dilution of SPC-1361 was sufficient for detection of c-Jun (pSer63) in 10 µg of mouse fibroblast cell line (nih 3t3) lysates by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Domain Families, Hlh, Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, SMADs, Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Transcription Factors, Cancer, Tumor Biomarkers, Oncoproteins, Oncoprotein Transcription Factors, Immunology, Innate Immunity, TLR Signaling
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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